nick translation वाक्य
उदाहरण वाक्य
मोबाइल
- Tagging can be done in various ways, such as nick translation, or PCR using tagged nucleotides.
- This process is called nick translation because the DNA to be processed is treated with DNase to produce single-stranded " nicks ".
- The structural and chemical aspects of this process, generally referred to as'nick translation', exceed the scope of this article.
- "' Nb . BbvCI "'is a fluorochrome-labeled nucleotides into specific spots of a DNA sequence via nick translation.
- Nick translation could cause double-stranded DNA breaks, if DNA polymerase I encounters another nick on the opposite strand, resulting in two shorter fragments.
- Nick translation is a biological process in which a single stranded DNA nick serves as the marker for DNA polymerase to excise and replace possibly damaged nucleotides.
- They can be used for strand-displacement amplification, Nicking Enzyme Amplification Reaction, exonucleotyic degradation, the creation of small gaps, or nick translation.
- Nick translation is used to label the DNA and involves cutting DNA and substituting nucleotides labelled with fluorophores ( direct labelling ) or biotin or oxigenin to have fluophore conjugated antibodies added later ( indirect labelling ).
- A DNA polymerase may perform this replacement via nick translation, a terminal excision reaction by its 5'--> 3'exonuclease activity, followed by a fill-in reaction by its polymerase activity.
- Therefore, the interpretation of these " in situ " assays of DNA fragmentation " in situ " nick translation ( ISNT ), TUNEL assay must be carefully assessed together with morphological features of apoptotic cells.
- In " DNA Pol I is the enzyme responsible for replacing RNA primers with DNA . DNA Pol I has a 5'to 3'exonuclease activity in addition to its polymerase activity, and uses its exonuclease activity to degrade the RNA primers ahead of it as it extends the DNA strand behind it, in a process called nick translation.
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